A Laboratory Manual of Physical Pharmaceutics is introduced to the B.Pharm students for easy understanding of the principles of physical pharmaceutics. The Experimental manual covers experiments to provide fundamental principles of physical pharmacy necessary to design physically and chemically stable dosage forms and ensure their therapeutic safety and efficacy. This manual is a unique in nature as it covers the two necessities of students: text on theoretical principles and its application including illustrative exercises in the form of practical.

     ·      This Book illustrates all the experiments included in various Universities syllabus of physical pharmacy.

     ·      It also provides an integrated understanding of theory and practical applications associated with physicochemical concepts in a very lucid language.

     ·      Reviews the physico-chemical concepts in the design of various dosage forms.

     ·      Provides several experiments related to physical chemical characteristics of any dosage forms.

Contents:

Basic Rules and Requirements

1. Pharmaceutical Microbiology and Basic Rules of Laboratory

2. Basic Requirements of Microbiology Laboratory

Instrumentation

3. Microscope

4. Autoclave

5. Incubator

6. Hot Air Oven

7. Laminar Air Flow

8. pH Meter

9. Spectrophotometer

10. Colony Counter

Preparation of Culture Media, Sterilization and Culture Methods

11. Preparation and Sterilization of Media

12. Aseptic Technique and Transfer of Microorganisms

13. Growth Promotion Test of the Medium

14. Preparation of Agar Slants, Stabs and Plates

15. Preservation of Microbial Cultures

Techniques for Isolation of Pure Culture from Mixed Culture

16. Streak Plate Method

17. Pour Plate Method

18. Spread Plate Technique

Staining Techniques of Microorganisms

19. Preparation of a Bacterial Smear

20. Staining

21. Simple Staining

22. Negative Staining

23. Gram Staining
24. Acid-Fast Staining

25. Staining of Fungus by Lactophenol Cotton Blue

Biochemical Tests for Identification of Bacteria

26. IMViC Tests

27. Indole Test

28. Methyl Red and Voges-Proskauer Test (MR-VP Test)

29. Citrate Test

30. Carbohydrate Fermentation Test

31. Starch Hydrolysis Test

32. Gelatin Hydrolysis Test

33. H2S Production Test

Bacterial Motility

34. Wet Mount Method

35. Hanging Drop Method

36. Soft Agar and Agar Deep Tube Methods

Microbial Assay of Antibiotics

37. Microbial Assay of Antibiotics

38. Turbidimetric or Tube Assay Method

Sterility Testing of Pharmaceuticals

39. Sterility Testing of Pharmaceuticals

Environmental Monitoring

40. Environmental Monitoring by Settling Plate Method

Bacterial Analysis of Water

41. Bacterial Examination of Water by Multiple Tube Fermentation Test

42. Bacteriological Examination of Water by Membrane Filtration Method

43. Analysis of Water for Total Bacterial Population by Standard Plate Count

Microbial Taxonomy

44. Fungi

About the Author:

Sadhan Kumar Dutta, M.Sc, PhD., F.I.C., started his career as a lecturer in Chemistry at Rajshahi Govt. College, Foujdarhat Cadet College, Sylhet Govt. College and also worked as lecturer in Biophysical Chemistry in the Department of Biochemistry of Dacca University. He had joined as a lecturer in Pharmaceutical Chemistry at the Department of Pharmaceutical Technology, Jadavpur University, Kolkata and served the university for 35 years and finally retired as professor of Pharmacy. In between he also served M.S. University of Baroda as a Professor of Pharmaceutical Technology. He also served as Emeritus Professor at Gupta College of Technological Sciences, Asansol, Bengal School of Technology, Sugandha and Seemanta Institute of Pharmaceutical Sciences, Orisa. He has authored over 60 research papers and still active as research guide. He has authored a well reputed book “Principles of Physical Chemistry and Biophysical Chemistry” for the students of physical chemistry, physical pharmaceutics and biochemistry. He is the recipient of many awards like 2000 outstanding people of 20th century' etc.